치아재식후 치유과정에서 fibronectin의 분포에 관한 면역조직화학적 연구
Immunohistochemical Distribution of Fibronectin, Cell Adhesion Glycoprotein During Repair of Rat Replantation Model
윤동호, 조진호, 김명식, 정희숙,
소속 상세정보
윤동호 ( ) - 경희대학교
조진호 ( ) - 경희대학교
김명식 ( ) - 경희대학교
정희숙 ( ) - 경희대학교
KMID : 0355819950190010065
Abstract
Periodontal healing requires the formation of new periodontal tissues comprised of various extracellular components, The present study investigated the formation and distribution of the fibronectin FN), a major adhesive glycoprotein Rats weighing
130~150 gm were fed 0.4% β-aminopropionitrile for 5 days to achieve gentle tooth extraction. The maxillary first molars were extracted under anesthesia with pentobarbital, washed in sterile distilled water, treated with bacterial collagenase to
digest
collagen fibers on the root surfaces. After washing in water overnight, the mesial root, surfaces were demineralized by application of citric acid, washed, dried and stored at 4℃.
Immediately after tooth extraction and bleeding control, the treated molars extracted previously from other rate were reimplanted.
Ten animals each were sacrificed 45 min, 1, 3, 6, 10 days after reimplantation of teeth by intracardiac perfusion with 4% paraformaldehyde. The replanted molars and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin.
Immunostaining of the FN was achieved by the avidin-biotin complex method.
@ES The results as follows ;
@EN 1. The control group showed gradual healing from 1 day and complete regeneration of the periodontium at 10 days.
2. At early healing stage, intense staining for FN was observed in the newly formed connective tissues, graulation tissue, especially around blood vessels as well as inflammatory cells.
3. At later healing stage, FN was expressed later in the periodontal ligament, cementum, osteoid, and newly formed connective tissue and its attachment site to the root surface.
4. Strong immunolabelling for FN was found in cementoblasts, osteoblasts, and new bone and cementum, which weaken staining was observed on the dentin and alveolar bone.
5. These results suggest that the healing after replantation is achieved through a series of cell differentiation and formation of FN.
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